Many molecules in the TRIM protein family participate in the occurrence and development of osteosarcoma through the role of E3 ubiquitin ligases; MDM2 and CRLs regulate the expression of P53 and P21 molecules to participate in the proliferation, migration, and invasion of osteosarcoma; RAD18 and RNF8 play a role in tumor drug resistance mechanisms by participating in DNA damage repair. The gene discussed is RNF8; the disease is osteosarcoma.