In order to confirm whether lumbrokinase down-regulates VEGF expression by acting on BPTF, as our previous research reported that VEGF was regulated by BPTF in NSCLC [36], we first analyzed the binding of BPTF at the VEGF promoter region by DNA pull-down assay using the biotin-labeled VEGF promoter probe, which was equivalent to promoter region −1157 to −1667 of the VEGF gene (Figure 3A). Here, VEGFA is linked to non-small cell lung carcinoma.