In this setting, CCl4 was administered for 12 weeks after the tail vein injection of Cre-expressing adenovirus (adeno-Cre) in Stk4(−/−)Stk3(F/−) (also known as Mst1(−/−)Mst2(F/−); F indicates a floxed allele) mice, and it resulted in the development of HCC tumors concomitantly with liver cirrhosis [50]. Here, STK3 is linked to hepatocellular carcinoma.