The multivariate analysis revealed that high BubR1 expression was independently associated with short survival; the hazard ratio (HR) was 1.568 (95% CI 1.075–2.287) compared to the low BubR1 expression group, as shown in Table 2 (p = 0.02), which indicates that BubR1 is an independent prognostic factor for the short survival of patients with CCA. This evidence concerns the gene BUB1B and cholangiocarcinoma.