Hypoxia significantly increased the binding and uptake of [177Lu]Lu-PSMA in the MCF-10A (0.3% vs. 3%) and MDA-MB-231 (0.4% vs. 3.4%) lines, whereas the prostate cancer cells (also studied for comparison purpose) showed decreased uptake under hypoxic conditions. The gene discussed is FOLH1; the disease is prostate carcinoma.