Given the pivotal role of renal TECs in I/R-AKI, we specifically focused on the proximal tubular epithelial cell cluster (PT) and further categorized it into three subtypes, including Damage-PT (identified by the marker genes Ccl2 and Havcr1), Normal-PT (characterized by the marker genes Lrp2 and Slc34a1) and Repair-PT (defined by the marker genes Mki67 and Top2a) (Fig. 1C-E). This evidence concerns the gene TOP2A and acute kidney injury.