Owing to the expression of TCF7L2 varying from different HCC cell lines (Figure S1B), we selected HepG2 and HCCLM3 cell lines for TCF7L2 knockdown experiments, SK-Hep-1 (abbreviated as SK1) and Huh7 cell lines for TCF7L2 overexpression experiments. Here, TCF7L2 is linked to hepatocellular carcinoma.