In order to examine the role of the Mst1–FoxO1–C/EBP-β pathway in mediating protection against myocardial injury during ischemia followed by reperfusion (I/R), another type of ischemic event that promotes myocardial injury, FoxO1-cKO mice, cardiac-specific FoxO1 overexpression (Tg-FoxO1) mice, and WT mice were subjected to 45 min of ischemia and 24 h of reperfusion. Here, FOXO1 is linked to ischemia.