To investigate the potential role(s) of autism-associated NLG3 in the MS related to sleep-wake and social behaviors, we generated CKO mice by injecting adeno-associated virus (pAAV-hSyn-Cre) into the MS of Nlg3fl/fl mice (Figure 1, A and B), resulting in the specific deletion of Nlg3 in the MS (Nlg3-CKO mice). This evidence concerns the gene RIC8B and autism.