Our previous study indicated that these biosensors could detect most Stx subtypes including those closely associated with HUS (Stx2a, 2c, and 2d), but failed to detect Stx2e and Stx2f (less commonly involved in human diseases); therefore, STEC strains producing these Stx2 subtypes will be missed. This evidence concerns the gene STX2 and hemolytic-uremic syndrome.