In this study, although NAFLD was not among the pathways significantly regulated by the DON group in this study, the phospholipase D signaling pathway is significantly regulated by the DON and 15-ADON groups, and PIK3R2, as a gene co-regulated in both the phospholipase D signaling pathway and the NAFLD pathway, served as a bridge linking the two pathways. The gene discussed is PIK3R2; the disease is metabolic dysfunction-associated steatotic liver disease.