Consequently, significantly higher NO bioavailability resulted from potentially lower ROS in the circulation as a result of the re-methylation action of betaine on Hcy, and the significantly lower expression of ACE that resulted as a counter-regulatory action of enhanced eNOS expression (manifested by the higher NO in the circulation) in the betaine-supplemented SHR rats might have acted independently and as well as integratively to ameliorate the high blood pressure. The gene discussed is ACE; the disease is hypertensive disorder.