The ability of insulin and inhibitors of autophagy—3-methyladenine (3-MA) and apoptosis (acetyl-aspartyl-glutamyl-valyl-aspartal—Ac-DEVD-CHO)—to change the levels of autophagic marker LC3B-II and glial fibrillary acidic protein (GFAP) increased in the hippocampus and frontal cerebral cortex during ischemia, and reperfusion was also studied. Here, INS is linked to ischemia.