To gain more insight into the biological function of APC-derived EVs in CRC, the expression of some ECM components, including fibronectin I (Fn1), collagen type I (Col1A1), and collagen type IV (Col4A1), was evaluated in the total mRNA from the fibroblasts after 72 h of treatment with EV-containing preparations (Figure 4). The gene discussed is COL4A1; the disease is colorectal carcinoma.