IGF1 and liver disorder: Finally, when comparing fish with ‘early’ and ‘advanced’ symptoms of liver disease, the major contributors to the dissimilarity between these groups (average dissimilarity 10.29%) were cyp7a1b, pparga, igf1, elovl5b, saa5, plasma AST activity, casp3a, tnxb, plasma LDH and CK activity, 5loxa, fish score, plasma [cortisol], and liver triacylglycerol levels, with the first four factors being significantly higher in fish with ‘early’ symptoms (Figure 4E, p-perm =0.0006).