Pathway enrichment based on GSEA of all marker genes and human Hallmark functional collection (FDR adjusted p-value < 0.25) showed downregulation of TNF-α signaling via NFKB in F_A in active vs. remission EoE with downregulation of the top leading edge genes JUN, EGR1, FOS, HES1, ZFP36, GADD45B, PPP1R15A, FOSB, JUNB, IER2, NR4A1, DUSP1, ATF3, MYC, and CEBPD (Figure 4b). The gene discussed is TNF; the disease is eosinophilic esophagitis.