RNA-seq analysis demonstrated the changes of cell differentiation and proliferation/apoptotic pathways (Figure 1—figure supplement 3) These findings indicate that the absence of non-core RAG regions accelerates malignant transformation and leukemic proliferation, leading to a more aggressive disease phenotype in the Ragc/c BCR-ABL1+ B-ALL mouse model. This evidence concerns the gene RRAGC and precursor B-cell acute lymphoblastic leukemia.