IFNB1 and neoplasm: Both the YUMM5.2 Ifnb1eGFP and B16-F10 Ifnb1eGFP cell lines showed reliant Dox-induced IFNβ-secretion both in vitro and in vivo (Figs. 1A–E, 3A and B) and in vivo Ifnb1eGFP tumor cell–associated macrophage phenotypes could be accurately reproduced using the in vitro BMDM system including INFβ exposure released from induced tumor cells and by means of recombinant protein (Figs. 2G–L, 3H and I).