To investigate if tumoral IFNβ shapes the TME, we performed flow cytometry analysis of infiltrating immune cells from established YUMM5.2 Ifnb1eGFP brain tumors after late Dox diet, allowing tumors to grow and yield sufficient tumor-infiltrating cells for individual analysis (Supplementary Figs. S2B, S2C and S3A). This evidence concerns the gene IFNB1 and neoplasm.