Finally, RNA-Seq analysis within the Xiangya CRC validation cohort corroborated these findings, revealing consistent positive correlations between NMUR1 and various immune cell subtypes, such as CD8+ T cells and NK cells, as well as immune checkpoints such as CD244, LAG3, CD40, and PDCD1 (Figure 3D). Here, LAG3 is linked to colorectal carcinoma.