To investigate the functional changes in CD8 TILs, we performed intracellular cytokine staining to detect the percentage of IFN-γ+, TNF-α+ or IFN-γ+TNF-α+ CD8 T cells from TB splenic control or C-100 vs. C-225 tumors (Figures 3A-E). The gene discussed is TNF; the disease is tuberculosis.