In this study, we analyzed the physiological indexes of the mice and found that after 4 weeks of electroacupuncture treatment, the body weight, food intake, and water intake of mice in the Acu + AMPK antagonist group and the Acu + ACC antagonist group decreased compared with those in the first week, suggesting that electroacupuncture treatment improved the obesity and excessive food and water intake in T2DM with NAFLD mice. Here, PRKAB1 is linked to metabolic dysfunction-associated steatotic liver disease.