In this study, we established a mouse model of T2DM with NAFLD and included animals that met the model criteria in the experiment to study the physiological and pathological mechanisms of disorders of glucose-lipid metabolism by observing the body weight, food intake, water intake, changes in blood glucose and blood lipids, hepatic steatosis, as well as the protein and gene indexes related to the AMPK/ACC pathway in mice. The gene discussed is PRKAB1; the disease is metabolic dysfunction-associated steatotic liver disease.