Subsequently, we performed qRT–PCR for verification, and the results showed that after adding GluOC into MDA-MB-231 breast cancer cells, the levels of ROCK1, JAK2, PIK3CA, Bcl-2, CREB were significantly increased, while the expression levels of Bax were significantly decreased (Fig. 1f). The gene discussed is PIK3CA; the disease is breast carcinoma.