Our findings suggested that DSP-0509 combined with RT resulted in increased level of memory T cells as well as the activation of CTLs that recognize cancer antigens in mice with complete tumor elimination, suggesting that it may be reasonable for the anti-tumor response of mice to the combination to differ according to expression of PD-L1 and TIM3, which are markers of CTL exhaustion. This evidence concerns the gene HAVCR2 and cancer.