CD4 and neoplasm: CD8+T cells, CD4+T helper cells, and Tregs were closest located to each other (CD8+T cells to CD4+T helper cells 23 μm and to Tregs 33 μm) and to macrophages (30 μm), while tumor cells were situated further away (55 μm), and B cells even more so (67 μm, figure 4L).