TJP1 and stroke disorder: We observe a decrease in the TJ proteins ZO-1 and Occludin beginning between 6 and 12 hours post-stroke, followed by endothelial cell death at 12 and 24 hours, and a reduction in vessel length at 24 hours, potentially as a direct consequence of endothelial cell death.43 This delayed endothelial cell death aligns with prior studies indicating limited endothelial cell loss within the first 24 hours following stroke.17,44,45