We observe a decrease in the TJ proteins ZO-1 and Occludin beginning between 6 and 12 hours post-stroke, followed by endothelial cell death at 12 and 24 hours, and a reduction in vessel length at 24 hours, potentially as a direct consequence of endothelial cell death.43 This delayed endothelial cell death aligns with prior studies indicating limited endothelial cell loss within the first 24 hours following stroke.17,44,45. The gene discussed is TJP1; the disease is Stroke.