Furthermore, we demonstrated that Mg2+ weakened the interaction between DBN1 and ACTN4 by reducing the phosphorylation of DBN1 at S142 (DBN1S142p), which enhanced the interaction between ACTN4 and F-actin and promoted F-actin polymerization, ultimately leading to the down-regulation of MMP2 and reduced cancer cell migration. The gene discussed is ACTN4; the disease is cancer.