We hypothesized that particular Gardnerella species may be more associated with BV.<h4>Methods</h4>Quantitative polymerase chain reaction (PCR) assays were developed targeting the cpn60 gene of species groups including G. vaginalis, G. piotii/pickettii, G. swidsinskii/greenwoodii, and G. leopoldii. This evidence concerns the gene HSPD1 and bacterial vaginosis.