Moreover, we found that many types of immune cell infiltration and immunotherapy markers were positively correlated with CTHRC1 expression in colon cancer, thyroid cancer, and melanoma (macrophages, neutrophils, dendritic cells, CD4+ T cells, CD8+ T cells/CD274, PDCD1, CTLA4, LAG3 in colon cancer; neutrophils, dendritic cells, B cells, CD4+ T cells, macrophages/CD274, PDCD1, CTLA4, LAG3 in thyroid cancer; neutrophils, macrophages/CTLA4 in melanoma). Here, CD4 is linked to malignant colon neoplasm.