To further define the tissue of origin of zebrafish p53EPS CNS lesions, we co-injected linearized gfap:EGFP (Don et al., 2017) and visualized EGFP expression in 100% of brain masses at 30–40 dpf (Figure 1A and B), supporting glial identity and a novel in vivo model of malignant glioma. The gene discussed is GFAP; the disease is malignant glioma.