EGFR and brain neoplasm: To generate a patient-relevant brain tumor model in zebrafish, we used the zebrafish her4.1 promoter to simultaneously over-express constitutively active human EGFR (EGFRvIII) and PI3KCA (PI3KCAH1047R) variants in neural progenitors and radial glia of syngeneic tp53 loss-of-function mutant larvae (Ignatius et al., 2018; Takke et al., 1999; Than-Trong et al., 2020).