This rapidly leads to explosive rupture of the PV membrane (PVM) and RBC membrane to allow the release of invasive merozoites.11–14 A single orthologue of SUB1 is found in the genomes of all known Plasmodium species, and a critical role of SUB1 for parasite survival has been genetically confirmed through the demonstration that SUB1 gene disruption results in a complete block in merozoite egress in asexual blood stages of the parasite life cycle and the preceding liver stages of infection. This evidence concerns the gene SUB1 and infection.