Compared to conventional high‐throughput sequencing, PANDORA sequencing with AlkB and T4PNK enzyme treatment resulted in a significant increase in reads for tsRNAs and rsRNAs without significant alterations in miRNA profiles, which revealed a broader and more accurate composition of sncRNAs in a wide range of mouse and human tissues and cells,19 and has been applied to various diseases, including astheno‐teratozoospermia and atherosclerosis.35, 36. This evidence concerns the gene ALKBH1 and atherosclerosis.