Compared to conventional high‐throughput sequencing, PANDORA sequencing with AlkB and T4PNK enzyme treatment resulted in a significant increase in reads for tsRNAs and rsRNAs without significant alterations in miRNA profiles, which revealed a broader and more accurate composition of sncRNAs in a wide range of mouse and human tissues and cells,19 and has been applied to various diseases, including astheno‐teratozoospermia and atherosclerosis.35, 36. This evidence concerns the gene ALKBH1 and Abnormal sperm morphology.