XAF1 and renal cell adenocarcinoma: In 2006, Reu et al. assessed the dependence of IFN-induced apoptosis on the XAF1 gene methylation status by applying DNMT inhibition (treatment with decitabine) or DNMT depletion (transfection with antisense RNA) on the RCC cell lines ACHN and SK-RC-45 [15].