For both primary and secondary tumorsin Hepa1-6 and 4T1 tumor models, all mice treated with AMPNs and anti-PD-1alone demonstrated an increasing fraction of CD4+ and CD8+ T cells in TIME, with the largest increase in the AMPNs +anti-PD-1 group (Figures 8J,K and S44). This evidence concerns the gene CD8A and neoplasm.