In this study, we selected thioredoxin peroxidase (Ov-TPx-1), cathepsin F (Ov-CF-1) and calreticulin (Ov-CALR) proteins of O. viverrini were selected as literature has shown their role in inflammation and CCA development by antioxidant, ECM, and hemoglobin degradation, and host immune system modulating activities (66). Here, CALR is linked to cholangiocarcinoma.