Finally, we performed EphA2-VE-CAD fluorescence co-localization assay on HCC tissue sections, and found that there was consistency in the expression amount (same signal) and spatial location (supra/extra-membranous) between EphA2 and VE-CAD, suggesting that EphA2 was expressed simultaneously in the same amount and at the same location as VE-CAD, which also confirmed that there was a direct correlation between EphA2 and VM, thus confirming the direct correlation between EphA2 and VM. Here, EPHA2 is linked to hepatocellular carcinoma.