The results of Western blot analysis showed significant increases in the expressions of Nrf2, HO-1, TLR4, p-NF-κB/NF-κB, IL-1β, and IL-6 protein after VD induction in comparison with those of the sham group (P < 0.05, P < 0.01 respectively). The gene discussed is NFE2L2; the disease is sexually transmitted disease.