Verification of the mechanism of EXO‐PD‐L1‐HGF in the stroke model by means of RNA‐sequencing analysis revealed upregulation of FOXO3 mRNA expression in the EXO‐PD‐L1‐HGF‐treated group, verified the EXO‐PD‐L1‐HGF‐induced proliferation of NPCs, and recognized the involvement of the STAT3/FOXO3 pathway in the neuroprotective effects after MCA occlusion injury. The gene discussed is STAT3; the disease is Stroke.