To verify the neuroregenerative ability of EXO‐PD‐L1‐HGF through the activation of the FOXO3 pathway in mice with stroke, we performed infarct volume measurements by means of triphenyltetrazolium chloride (TTC) staining and neurological functional assessments of control, EXO (with FOXO3‐NL or FOXO3–/–)‐treated, and EXO‐PD‐L1‐HGF (with FOXO3‐NL or FOXO3–/–)‐treated ischemic stroke models. This evidence concerns the gene FOXO3 and ischemic stroke.