In summary, based on the results of in vitro and in vivo experiments, 3 h(incubation period) and 9 h(peak period) were selected as the critical points of this study, and a series of differential genes (mapk1, il1b, rela, nfkbiab, akt3a, hyou1, hsp90b1, dnajc3a et al.)and pathways (C-type lectin receptor signaling pathway, protein processing in the endoplasmic reticulum, Toll-like receptor signaling pathway, Salmonella infection et al.)caused by BF/C2 in response to GCRV infection were analyzed by transcriptome sequencing. The gene discussed is MAPK1; the disease is Salmonella Infections.