Whereas overexpression of CDK5RAP2 S down-regulated E-cadherin and up-regulated N-cadherin, Vimentin, Zeb1, Slug and Twist1 expression (Fig. 4B), indicating that CDK5RAP2 L and CDK5RAP2 S might have different roles in EMT of colon cancer cells. The gene discussed is TWIST1; the disease is malignant colon neoplasm.