By overexpression of CDK5RAP2 L or CDK5RAP2 S after CDK5RAP2 knockdown via lentiviral infection in SW480-luc cells (Supplementary Fig. S3A, B), we found that CDK5RAP2 S, but not CDK5RAP2 L promoted the migration and invasion of colon cancer cells (Fig. 4A). Here, CDK5RAP2 is linked to malignant colon neoplasm.