Infection of the cow mammary gland by pathogenic microbes causes an increase in leukocyte numbers in the breast, as well as the release of huge amounts of inflammatory cytokines, which cause neutrophil accumulation and activation.Blood samples of Xinjiang brown cattle with different SCCs were collected to plot the methylation patterns of CpG sites; BSP assays revealed that the DMRs of the TRAPPC9 gene were highly methylated. This evidence concerns the gene TRAPPC9 and infection.