By employing an autozygosity mapping approach (Lander and Botstein, 1987; Abecasis et al., 2002; Carr et al., 2011b; Al-Mutairi et al., 2022; Al-Mutairi et al., 2023), we identified two novel homozygous missense variants within DNAH5 in two PCD individuals from family KU-5 as well as compound heterozygous variants of DNAH5 in PCD individuals from families KU-2 and KU-9, all of which were responsible for the development of PCD. The gene discussed is DNAH5; the disease is primary ciliary dyskinesia.