Overall, our data suggests that NaV1.7 is uniquely upregulated in MTC and the inhibition of NaV1.7 by SV188 showed a use-dependent and a voltage-dependent blockade suggesting that SV188 enters the central cavity of the channel via the intracellular gate and bind within the permeation pathway of the pore channel. This evidence concerns the gene SCN9A and medullary thyroid gland carcinoma.