The precision of ReactomeGSA facilitated the generation of bar plots (Fig. 4B and C), which brought into focus the activities of specific pathways, notably “FGFR1c and Klotho ligand binding and activation” and “Hydroxycarboxylic acid-binding receptors.” These visualizations laid bare the disparate engagement of these pathways across macrophage subsets, offering a window into their potential functional differentiation within the context of renal disease. The gene discussed is KL; the disease is kidney disorder.