Dramatic reductions in leukocyte recruitment were observed in TNF KO mice in a time-dependent manner, where granulocytes were significantly decreased uniformly in the brain, galea, and bone flap of TNF KO mice at day 14 post-infection, both by relative abundance (Fig. 1D-F) and absolute count (Additional File 1: Fig. S2D-F). The gene discussed is TNF; the disease is infection.