Next, we used clinical tissue samples to analyze the correlation between USP53 expression and clinicopathological characteristics of breast cancer, and found that the level of USP53 was negatively correlated with the clinical stage of breast cancer, which was consistent with the results of the clinical correlation analysis of USP53 in the study of esophageal cancer by Cheng et al. [17]. This evidence concerns the gene USP53 and esophageal cancer.