This indicated that ZMYND11 knockdown reversed the effects of proliferation inhibition, G1 phase arrest, pro-apoptosis and mitochondrial damage in breast cancer cells caused by overexpression of USP53, thereby reverently confirming the in vitro inhibitory effect of USP53 deubiquitination stabilizing ZMYND11 expression. The gene discussed is ZMYND11; the disease is breast carcinoma.