Although qPCR detected the JAK2 V617F mutation in primary CML (0.02%) and CML-CR (1.54%) stages, the mutation burden detected is significantly below the qPCR sensitivity threshold of 3.40% (LoD) and lower compared to the values detected by cdPCR. The gene discussed is JAK2; the disease is chronic myelogenous leukemia, BCR-ABL1 positive.