IFNG and tuberculosis: Induction of trained immunity was assessed by the measurement of the cytokines IFNγ and IL1β secreted by PBMCs in response to stimulation with microorganisms unrelated to TB, such as Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Candida albicans (C. albicans) and lipopolysaccharide (LPS).