Conversely, HYAL2 expression was increased (Figure S7c, Supporting Information), similar to CD44 expression (Figure 2f), as previously observed in the tumor microenvironment.[41] Vascular glycocalyx degradation was further confirmed by a severe decrease in HA concentration in the periphery of SKBR3 and MDA‐MB‐468 tumoroids (between 0.5 mm and 1.5 mm from the tumoroids) compared to control MVNs (Figure 2g). Here, HYAL2 is linked to neoplasm.