Lastly, fecal microbiota transplantation from NaB-supplemented mice to BDL mice may be a possible strategy to confirm the beneficial effects of NaB on cholestasis through modulation of gut microbiota, even as this study shows that NaB administration could alleviate cholestasis via increasing FGF21 expression and regulating gut microbiota. Here, FGF21 is linked to cholestasis.