Stimulation with IFN-γ induces autophagy in cultured intestinal epithelial cells, a process that is partially impeded by knockdown of Nostrill, Igtp, iNos, or Gadd45g. These findings suggest that Nostrill may augment IFN-γ-mediated cell-intrinsic defense against Cryptosporidium infection in intestinal epithelial cells by promoting the transcription of genes associated with autophagy. This evidence concerns the gene GADD45G and cryptosporidiosis.